nir fluorescence emission spectra  (Ocean Optics)

Journal: Advanced Materials (Deerfield Beach, Fla.)

Article Title: Unlocking NIR‐II Photoluminescence in 2D Copper Tetrasilicate Nanosheets through Flame Spray Synthesis

doi: 10.1002/adma.202503159

Figure Lengend Snippet: Photoluminescence engineering of copper tetrasilicates enables emission shift to NIR‐II window. a) NIR emission spectrum of BaCuSi 4 O 10 and its mixed forms showing a significant impact of (multi)element doping toward shifting the emission into the NIR‐II window (> 1000 nm). b) Evaluation of the NIR emission spectra as integrated for NIR‐I (simplified as < 1000 nm) and for NIR‐II (> 1000 nm). c) Simplified energy diagram of Cu 2+ ion within a tetragonally distorted crystal field, for i) non‐doped, single M ‐containing NS and ii) multielement doped NS, highlighting the shifted E a energy levels iii). d) Absolute photoluminescence quantum yield (PL‐QY) spectra of CaCuSi 4 O 10 . Integrated photon counts within the gray box, excitation at 630 nm. e) PL‐QY dependency on the excitation wavelengths (red line = Gaussian fit; PL‐QY = 32%). f) PL‐QY engineering through variation of annealing temperature of resynthesized CTS. g) PL‐QY engineering through optimizing annealing time, showing a general trend of increasing PL‐QY with prolonged annealing (mean ± SD). h) Correlation between the lattice parameters a and c , obtained from Rietveld refinement, and the calculated (mean) ionic radius of the (mixed) alkaline earth metal CTS. i) Correlation of the optimized PL‐QY to the emission wavelengths of all synthesized 2D CTS variations (for comparison with reference values see Figure (Supporting Information), red line = second order polynomial fit). j) Correlation between the PL‐QY and fluorescence lifetime of all obtained materials (red line = second order polynomial fit of non‐Mg containing NS).

Article Snippet: In addition, NIR fluorescence emission spectra were recorded with a NIRQuest+1.7 spectrometer (slit width = 200 μm; InGaAs detector, OceanOptics), fiber‐coupled to a customized Axiovert 40CFL using a 10x objective, 800 nm dichroic mirror (Edmund optics) and 900 nm LP filter (FELH0900, Thorlabs).

Techniques: Synthesized, Comparison, Fluorescence

Journal: Advanced Materials (Deerfield Beach, Fla.)

Article Title: Unlocking NIR‐II Photoluminescence in 2D Copper Tetrasilicate Nanosheets through Flame Spray Synthesis

doi: 10.1002/adma.202503159

Figure Lengend Snippet: Nanosheet annealing through laser irradiation. a) Photograph of primary FSP particles (i; cyan) rearranged into NIR‐fluorescent SrCuSi 4 O 10 (ii; blue) through 808 nm laser irradiation (15.3 W cm −2 , white circle) (scale bar = 0.5 cm). b) Schematic representation of the in situ rearrangement process and XRD pattern of the corresponding particles. The amorphous primary FSP particles anneal within seconds into the characteristic P4/ncc tetragonal CTS crystal lattice, similar to a calcination process at 1000 °C (10 min). c) Fluorescence emission spectra of (multielement doped) CTS obtained by laser irradiation.

Article Snippet: In addition, NIR fluorescence emission spectra were recorded with a NIRQuest+1.7 spectrometer (slit width = 200 μm; InGaAs detector, OceanOptics), fiber‐coupled to a customized Axiovert 40CFL using a 10x objective, 800 nm dichroic mirror (Edmund optics) and 900 nm LP filter (FELH0900, Thorlabs).

Techniques: Irradiation, In Situ, Fluorescence

Journal: Advanced Materials (Deerfield Beach, Fla.)

Article Title: Unlocking NIR‐II Photoluminescence in 2D Copper Tetrasilicate Nanosheets through Flame Spray Synthesis

doi: 10.1002/adma.202503159

Figure Lengend Snippet: Engineered nanosheets for super‐resolution mapping of the murine brain. a) Schematic of the diffuse optical localization imaging (DOLI) system used for cerebrovascular imaging in the NIR window. A SWIR camera was used to collect the fluorescence emission of a dispersion of stabilized NS injected intravenously (i.v.) under 808 nm excitation (850 mW cm −2 ). b) Photostability of CTS NSs compared to a common organic dye (Rhodamine B). c) High‐frame‐rate imaging of CaCuSi 4 O 10 NS placed inside a vessel‐mimicking Teflon tube (280 µm inner diameter). Light scattering of brain tissues was mimicked with a 1.2% intralipid (IL) phantom (scale bar = 500 µm). d) Time‐lapse widefield images post DMSA‐stabilized NS injection (scale bar = 1 mm). e) Differentiation of veins and arteries based on their different perfusion patterns, distinguished through principal component analysis (PCA) (scale bar = 1 mm). f) Schematic overview i) of the working principle of DOLI rendering the structural ii), blood flow direction iii) and velocity iv, mm/s) maps of cerebral vasculature from continuous localization and tracking of circulating PEGylated NSs (scale bar = 1 mm).

Article Snippet: In addition, NIR fluorescence emission spectra were recorded with a NIRQuest+1.7 spectrometer (slit width = 200 μm; InGaAs detector, OceanOptics), fiber‐coupled to a customized Axiovert 40CFL using a 10x objective, 800 nm dichroic mirror (Edmund optics) and 900 nm LP filter (FELH0900, Thorlabs).

Techniques: Imaging, Fluorescence, Dispersion, Injection

Journal: Advanced Materials (Deerfield Beach, Fla.)

Article Title: Unlocking NIR‐II Photoluminescence in 2D Copper Tetrasilicate Nanosheets through Flame Spray Synthesis

doi: 10.1002/adma.202503159

Figure Lengend Snippet: Individual macrophage tracking in vivo. a) Macrophage cell toxicity test for various NS compared to SiO 2 (Aerosil 90; mean ± SD). b) Schematic representation of NSs uptaken by human macrophages, with respective bright field (BF) and NIR‐fluorescence images of a single NS‐labeled cell. c) Overlay of all tracked macrophages (N = 15) resemble parts of the vasculature tree (DOLI image from Figure , Supporting Information; scale bar = 1 mm).

Article Snippet: In addition, NIR fluorescence emission spectra were recorded with a NIRQuest+1.7 spectrometer (slit width = 200 μm; InGaAs detector, OceanOptics), fiber‐coupled to a customized Axiovert 40CFL using a 10x objective, 800 nm dichroic mirror (Edmund optics) and 900 nm LP filter (FELH0900, Thorlabs).

Techniques: In Vivo, Fluorescence, Labeling

Journal: Advanced Healthcare Materials

Article Title: Material‐Intrinsic NIR‐Fluorescence Enables Image‐Guided Surgery for Ceramic Fracture Removal

doi: 10.1002/adhm.202302950

Figure Lengend Snippet: Schematic of NIR‐image‐guided surgery for the removal of fractured hip implant ceramics. a) Hip arthroplasty, usually implanting ceramic heads, is a highly common surgery; however, up to 0.03% of implants will fracture in their lifetime, causing severe damage and the need for urgent intervention. b) Exemplary workflow of the NIR‐fluorescence‐based fracture removal. Fragments of ceramic implants remain within the tissue (I), which makes localization and hence complete removal challenging. Camera‐based visualization of the ceramic splinters (II) allows detection through tissue based on the ceramic‐intrinsic fluorescent properties in the NIR‐I biological transparency window. Hence, ceramic splinters and debris can be removed (III), resulting in ceramic debris‐free tissue (IV). c) Image of commonly used ceramic implant femoral heads and splinters. Golden‐ Biolox forte; white‐ Ceramys; pink‐ Biolox delta. d) Fluorescence spectra of the four tested ceramic implant materials show the strongest emission around 700 nm for Biolox delta. Analysis was performed with a standard silicon‐based detector optimized for the UV‐vis region. e) A specialized InGaAs detector reveals a prominent NIR‐fluorescence emission tail for Biolox delta.

Article Snippet: NIR fluorescence emission spectra were recorded with a Shamrock 193i spectrometer equipped with an InGaAs detector (Andor Technology Ltd., Belfast, Northern Ireland) using a 561 nm Cobolt Jive laser (Cobolt AB, Solna, Sweden) as excitation source.

Techniques: Fluorescence

Journal: Advanced Healthcare Materials

Article Title: Material‐Intrinsic NIR‐Fluorescence Enables Image‐Guided Surgery for Ceramic Fracture Removal

doi: 10.1002/adhm.202302950

Figure Lengend Snippet: Synthesis of NIR‐luminescent ZTA ceramics. a) XRD analysis of ZTA ceramics synthesized via FSP and temperature‐dependent calcination. Rietveld refinement of the 1400 °C sintered sample revealed the desired composition of 80% Al 2 O 3 and 20% ZrO 2 . b) Fluorescence spectra (upper panel, left) of ZTA sintered at 1200 °C show a Cr‐content‐dependent emission intensity (Cr [%] given as theoretical Cr 2 O 3 content). Differences in the fluorescence profile become visible in the normalized fluorescence emission spectra (upper panel, right). Fluorescence spectra (lower panel, left) of ZTA sintered at 1400 °C follow a Cr‐dependent emission intensity and increasing NIR tail (>700 nm) as seen in the normalized fluorescence emission spectra (lower panel, right). c) Excitation–emission maps of ZTA doped 1% Cr sintered at 1200 °C (left) and 1400 °C (right). d) Normalized fluorescence emission of commercial ZTA ceramic implant Biolox delta and synthesized ZTA with 0.5% Cr sintered at 1400 °C. e) PL‐QY highly depends on the Cr content (theoretical wt% Cr 2 O 3 in ZTA) as well as the sintering temperature (crystal/particle size). f) Photographs of ZTA powder show an increasingly strong pink color with increasing Cr content. Secondary electron (SE) image visualizes the morphology of sintered Al 2 O 3 particles (1400 °C), while the corresponding EDX spectrum g) provides information on the main chemical constituents. Insert shows the histogram size distribution of the Al 2 O 3 particles obtained from particle‐size analysis based on SE images ( N = 100).

Article Snippet: NIR fluorescence emission spectra were recorded with a Shamrock 193i spectrometer equipped with an InGaAs detector (Andor Technology Ltd., Belfast, Northern Ireland) using a 561 nm Cobolt Jive laser (Cobolt AB, Solna, Sweden) as excitation source.

Techniques: Synthesized, Fluorescence, Particle Size Analysis

Journal: Advanced Healthcare Materials

Article Title: Material‐Intrinsic NIR‐Fluorescence Enables Image‐Guided Surgery for Ceramic Fracture Removal

doi: 10.1002/adhm.202302950

Figure Lengend Snippet: Remote NIR fluorescence detection of ceramic splinters. a) Optical setup for camera‐based ceramic implant splinter identification (20 cm sample distance). Visible (Vis) photograph of the four ceramic types and their corresponding NIR‐fluorescence image through 2 mm of tissue phantom. b) Schematic representation of the measurement conditions, evaluating the optimal detection method, as well as its capabilities to detect ceramic fragments of different sizes through avian and bovine tissue phantom. c) Representative series of a 17 mm large delta fragment imaged through avian tissue phantom (532 nm excitation, 700 nm LP, CMOS camera). d) Evaluated exposure time to reach a relative intensity value ( I = 20, 8‐bit image), as a threshold for distinct fluorescence identification. A combination of varying the camera, excitation light source, LP emission filter, and tissue phantom (thicknesses) is indicated before exceeding a maximal integration time of 10 s. e) Proof‐of‐principle detection of Biolox delta fragments using a smartphone camera and a 650 nm LP filter i) without and ii) through 2 mm of avian phantom. f) Evaluation of detectable fragment sizes through 2 mm of avian phantom (InGaAs camera, 1 s integration time, 700 nm LP) and in (g) for smaller fragments through bovine tissue (638 nm excitation). h) Maximal penetration depth through avian and bovine tissue phantom versus Biolox delta ceramic fragment size for the tested InGaAs camera. i) Schematic for arthroscopy‐based hip arthroscopy examination. j) Setup for the arthroscopy prototype where an image‐guided fiber transfers the fluorescence image to a CMOS camera. k) Fluorescence images captured via the image‐guided arthroscopy fiber. i) Image of a 0.8 × 0.8 mm sized Biolox delta splinter. ii) Same splinter under 3 mm of chicken tissue phantom. iii) Background correction and thresholding clearly identify the ceramic splinter. iv) Fluorescence image of the splinter under 4 mm of chicken tissue phantom. l) i) Fluorescence image of the splinter under 4 mm bovine‐phantom. ii) 50–70 µm sized ceramic debris (while arrows) visualized using image‐guided arthroscopy.

Article Snippet: NIR fluorescence emission spectra were recorded with a Shamrock 193i spectrometer equipped with an InGaAs detector (Andor Technology Ltd., Belfast, Northern Ireland) using a 561 nm Cobolt Jive laser (Cobolt AB, Solna, Sweden) as excitation source.

Techniques: Fluorescence

Journal: Advanced Healthcare Materials

Article Title: Material‐Intrinsic NIR‐Fluorescence Enables Image‐Guided Surgery for Ceramic Fracture Removal

doi: 10.1002/adhm.202302950

Figure Lengend Snippet: Ceramic debris detection using NIR fluorescence microscopy. a) Modification of a commercial fluorescence microscope allows for the detection of Biolox delta ceramic particles. Excitation light is filtered using a 650 nm SP filter and reflected using a 638 nm cut‐on dichroic mirror toward the sample. The emitted light is transmitted through a 650 nm LP filter until it is detected by a standard CCD camera. b) Proof‐of‐concept visualization of ceramic debris in artificial synovia background containing fixed, stained fibroblast cells. i) Brightfield; ii) Alexa Fluor 488 staining; iii) DAPI staining; iv) ceramic fluorescence. c) Detection of ceramic debris in a blood background. i) Brightfield (with blood platelets); ii) ceramic fluorescence.

Article Snippet: NIR fluorescence emission spectra were recorded with a Shamrock 193i spectrometer equipped with an InGaAs detector (Andor Technology Ltd., Belfast, Northern Ireland) using a 561 nm Cobolt Jive laser (Cobolt AB, Solna, Sweden) as excitation source.

Techniques: Fluorescence, Microscopy, Modification, Staining

Journal: Analytical Chemistry

Article Title: Antibody-Free Rapid Detection of SARS-CoV-2 Proteins Using Corona Phase Molecular Recognition to Accelerate Development Time

doi: 10.1021/acs.analchem.1c02889

Figure Lengend Snippet: SARS-CoV-2 protein nanosensor library. (a) PEG-phospholipid library for CoPhMoRe-based SWCNT nanosensors. (b) Accessible surface area of the PEG-phospholipid wrapped SWCNTs, where q is the vacant binding site on SWCNTs, and K d is the dissociation constant of probe binding to SWCNTs. Assuming an equivalent probe binding strength K d , a higher q / K d value represents more accessible surface area and less corona phase coverage. (c) UV–vis–nIR absorption spectrum of PEG-phospholipid/SWCNT nanosensor library with distinct E 11 and E 22 transitions. (d) nIR fluorescence spectrum of PEG-phospholipid/SWCNT nanosensor library under 785 nm excitation.

Article Snippet: Figure c shows the UV–vis–nIR absorption spectrum for the PEG-phospholipid corona phase, where the distinct and sharp peaks of E 11 and E 22 transitions indicate the successful isolation and suspension of individual SWCNT. nIR fluorescent emission spectra ( Figure d) under 785 nm laser excitation demonstrate that nanosensors are mainly composed of (6, 5), (7, 6), and (9, 4) nanotube chirality.

Techniques: Binding Assay, Fluorescence